Service

Flow Cytometry and DNA Sequenzing Facility

Currently available applications include:

• cloning and cell sorting
• multi-color immunofluorescence
• DNA cell cycle analysis
• measurements of apoptosis
• calcium flux

The facility is equipped with the most advanced, state-of-the-art flow cytometers. For sorting, we have two Becton Dickinson (BD) Symphony S6 sorters, two AriaFusion (BD), one FACSAria III (BD), one Beckman Coulter MoFlo XDP sorter and a Miltenyi autoMACS magnetic sorter. For analysis of cell samples our facility provides one Symphony A5, three Fortessa and one LSR III (all from BD). The Sorters are versatile high speed sorters equipped with up to five lasers, enabling the measurement of up to thirthy fluorescent parameters.

All flow cytometers can simultaneously sort up to six populations at 30 000 cells per second with greater than 99,8% purity and high recovery. Selected populations can be sorted into test tubes or deposited directly into 384-well plates. Sterile cell sorting is available upon request allowing subsequent culturing of sorted cells. The magnetic cell separation system (autoMACS) can isolate large numbers of cells for a single surface marker with high purity in a short period of time. The Cell-Sorters are available on weekdays upon special arrangement with the operators. These instruments are often booked weeks in advance.