Head of Facility

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Dr. Ulrike Bönisch
Phone:+49 761 5108 697

Selected Publications

1.
Arrigoni L, Richter AS, Betancourt E, Bruder K, Diehl S, Manke T and Bönisch U (2015)
Standardizing chromatin research: a simple and universal method for ChIP-seq.
2.
Bönisch U, Arrigoni L, Betancourt E and Bruder K (2015)
Automatisierte Herstellung von ChIP-Seq-Sequenzbibliotheken
3.
Roidl D, Hellbach N, Bovio P, Villarreal A, Heidrich S, Nestel S, Grüning B, Bönisch U and Vogel T (2015)
DOT1L activity promotes proliferation and protects cortical neural stem cells from activation of ATF4-DDIT3-mediated ER stress in vitro.
4.
Mayer S, Gilsbach R, Preissl S, Monroy Ordonez EB, Schnick T, Beetz N, Lother A, Rommel C, Ihle HM, Bugger H, Rühle F, Schrepper A, Schwarzer M, Heilmann C8, Bönisch U, Gupta SK, Wilpert J, Kretz O, von Elverfeldt D, Orth J, Aktories K, Beyersdorf F, Bode C, Stiller B, Krüger M, Thum T, Doenst T, Stoll M, Hein L. (2015)
Adrenergic Repression of the Epigenetic Reader MeCP2 Facilitates Cardiac Adaptation in Chronic Heart Failure.
5.
Ost A, Lempradl A, Casas E, Weigert M, Tiko T, Deniz M, Pantano L, Boenisch U, Itskov PM, Stoeckius M, Ruf M, Rajewsky N, Reuter G, Iovino N, Ribeiro C, Alenius M, Heyne S, Vavouri T, Pospisilik JA. (2014)
Paternal diet defines offspring chromatin state and intergenerational obesity.
6.
Gilsbach R, Preissl S, Grüning BA, Schnick T, Burger L, Benes V, Würch A, Bönisch U, Günther S, Backofen R, Fleischmann BK, Schübeler D, Hein L. (2014)
Dynamic DNA methylation orchestrates cardiomyocyte development, maturation and disease.

Deep Sequencing Facility

Deep Sequencing Facility

The deep sequencing unit is an sequencing service centre at the MPI-IE and offers high throughput sequencing services mainly for in house research groups. The facility is equipped with modern state of the art technology, highly qualified staff and interacts closely with the bioinformatics unit at the MPI-IE. Next to standard sample processing huge effort is put into the optimization, automation and standardization of existing workflows according to internationally emerging standards.

A robust instrumentation infrastructure is in place for standardized and quality controlled sample preparation. This includes a liquid handling stations for automated chromatin immunoprecipitation and library preparation. Zoom Image
A robust instrumentation infrastructure is in place for standardized and quality controlled sample preparation. This includes a liquid handling stations for automated chromatin immunoprecipitation and library preparation. [less]

Apart from serving all groups at the institute, the unit is also part of large German-wide consortia (MEDEP and DEEP) for which it produces ChIP-seq data and reference epigenomes from various tissue types. As one of the six epigenome production centers in Germany, the unit has set-up a complete semi-automated ChIP-Seq pipeline that enables chromatin extraction, chromatin immunoprecipitation, library preparation and deep sequencing according to highest international standards and quality controls.

Training is an important aspect of our work in tutorials the sequencing technology is explained furthermore users are advised on sample- and library preparation techniques.

 
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