Chromosome dynamics and gene expression
It is becoming increasingly clear that chromosomal organization as well as gene positioning has the potential to influence gene expression. X chromosome provides a nice example of a chromosome that is decorated with a ribonucleoprotein complex and is transcriptionally upregulated. We are interested in understanding how X chromosomal genes are organized within the X chromosomal territory but also within the nucleus with respect to the nuclear periphery to study whether and how this influences X-linked gene expression. We employ a multifaceted approach combining cell biology biochemistry and genetics to gain novel insights into the role of genome organization and gene expression. Our recent work employing chromosome conformation techniques (HiC and 4C) and high resolution imaging indicates that high affinity sites located on the X chromosome form an interaction network to facilitate dosage compensation.