The tissue resident memory (TRM) cells reside in non-lymphoid tissues where many of these organs, such as the skin and intestinal tract, are constantly exposed to external stimuli. TRM cells are therefore an essential part of tissue immunity for their localization enables more immediate and localized adaptive immune responses in comparison to circulating memory cells. While the signals required to reproduce canonical memory and effector T cell phenotypes in vitro are well-studied, the signals required to generate TRM cells in vitro are still unknown. My project seeks to establish culture systems that enable in vitro generation of CD8+ TRM cells. Establishing such culture conditions will not only provide a powerful tool for investigating TRM cell biology, but also potentially benefit therapeutic strategies.