Technologies and services

An inverted multi-modal system which has PALM&dSTORM super-resolution, SIM super-resolution, total internal reflection fluorescence (TIRF), and confocal (LSM) imaging modes. This permits, for instance, images of the same areas of interest both at sub-diffraction resolution (<100nm, super-resolution) and in LSM mode, which allows for flexibility of detection settings and large fields of view. (Instrument of Akhtar Department)

An inverted Nipkow spinning disc confocal microscope enables high frame rate and/or long-term observations of live samples with little photodamage, as well as fast acquisition of 3D datasets with efficient optical sectioning.

Inverted laser scanning confocal microscope, a versatile "work horse” system with multiple laser lines spanning the whole visible light spectrum and “tunable” spectral detectors, thus distinguishing signals from multiple fluorophores with overlapping spectra. This gives the users the full flexibility in the choice and combinations of multiple fluorescent labels in the same sample. Controling the illumination power in each pixel of the field of view makes this microscope ideally suited for photoperturbation techniques (such as FRAP, FLIP, photoactivation, etc.) which provide information about the dynamics of molecules and subcellular components in living cells.

Inverted combined multi-photon and confocal system, which can acquire high-quality images >100 micron deep in intact tissue samples. In addition, all functionalities of LSM780 confocal are possible.

A light sheet fluorescence microscope with built-in incubation chamber. Its light path configuration (illumination perpendicular to detection), dual-side illumination, and sample rotation permit fast 3D image acquisition of very thick (mm-scale) samples with extremely low photodamage. The instrument is suitable for (living) samples in water and optically cleared tissues in refractive index-matching media with RI from 1.38 to 1.45. (Instrument of the Akhtar Department)

An inverted microscope for epifluorescence and transmitted light imaging. The instrument is most suitable for time-lapse series when high resolution in z is not needed (e.g., cell migration). The FLUCS module (Cissé lab) coupled to that microscope permits the creation of directed intracellular flows on micron scale in a non-invasive way.

C-Trap instrument (Lumicks) combines optical tweezers, a confocal microscope, and microfluidics. Optical tweezers enable precise, non-invasive manipulation of microscopic objects, such as individual biological macromolecules and their associates in solution, as well as measurements of mechanical forces at the single molecule scale. Confocal fluorescent microscope permits real-time observations of these microscopic objects.

A versatile commercial software package for the visualization and analysis of multidimensional images, including very large datasets (>10GB). Multiple modules permit various tasks, such as manual and automatic segmentation (also machine learning-based), object tracking, surface rendering, particle analysis, and 3D tile stitching.

Advanced commercial software for image restoration based on deconvolution algorithms, with which users can recover image information of objects from various types of fluorescence images affected by out-of-focus light blurring and electronic noise. The software includes other image analysis tools (tiles stitching, multiple view fusion, aberration corrections, etc).

The most popular and versatile open-source scientific image processing software, for which dozens of plugins have been created by the scientific community to address a great variety of image analysis tasks.