Service

Flow Cytometry and DNA Sequenzing Facility

Currently available applications include

• Cloning and cell sorting
• Multi-color immunofluorescence
• DNA cell cycle analysis
• Measurements of apoptosis
• Calcium flux

The facility is equipped with modern flow cytometers that are equipped with a wide range of lasers and enable up to 30 fluorescent parameters. For sorting, we have two Becton Dickinson (BD) Symphony S6 sorters, two AriaFusion (BD), one FACSAria III (BD), one Union Biometra Biosorter, and one Miltenyi autoMACS magnetic sorter. For analysis of cell samples, our facility provides one Symphony A5 (BD), two Fortessa (BD), and one imagestream.

All flow cytometers can simultaneously sort up to six populations at up to 30,000 cells per second with greater than 99.8% purity and high recovery. Selected populations can be sorted into test tubes or deposited directly into 96-well or 384-well plates. Sterile cell sorting is available upon request, allowing subsequent culturing of sorted cells. The magnetic cell separation system (autoMACS) can isolate large numbers of cells for a single surface marker with high purity in a short period of time. Please contact facility staff for booking a sort or if you have any questions.