Fish Facility


The fish facility at the Max Planck Institute of Immunobiology and Epigenetics was opened in 2007. It houses zebrafish (Danio rerio) and Medaka (Oryzias latipes) fish. Each room is equipped with an independent water treatment unit. Access to the facility is restricted and automatically recorded; technical parameters of the entire facility are remotely controlled. A separate quarantine room provides opportunity for short-term experiments without compromising the high hygienic standards of the main facility. Currently, about 50 different lines carrying ENU-induced mutations affecting different developmental processes and several transgenic lines are kept in the system. Wild-type strains are maintained for breeding experiments as well as for general egg and embryo supply. The facility is run by a group of four staff members.

The following procedures are routinely used:

- Pressure-driven microinjection of mRNA, DNA, or antisense morpholino oligonucleotides into fertilized eggs

- BAC transgenesis

- Micropoint-laser assisted uncaging of fluorescent dyes, cell ablation, or heat-shock promoter-driven transgene expression in single cells

- Cell transplantations in embryos and adult fish

- Automated whole mount in situ hybridisation

- Sperm cryoconservation

- Homozygosity mapping/positional cloning

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