MOF is a histone acetyl transferase with a central role in dosage compensation and part of two different protein complexes. The MSL and NSL complexes are conserved from Drosophila to mammals, and have been found to acetylate H4. In the MSL complex MOF acetylates H4K16. The MSL complex has been shown to facilitate dosage compensation in male flies, transcriptionally up regulating the single male X-chromosome to compensate gene dosage relative to the female X-chromosomes. My work aims to characterize and quantify how the MSL complex modulates transcription and identify how MOF regulates transcription. For this I will use a mixture of genomic and transcriptomic approaches to investigate the effects of dosage compensation genome wide in vivo, and look at how dosage compensation is achieved.